Fáilte chuig an Institiúid um Leigheas Athghiniúnach
Welcome to the Regenerative Medicine Institute (REMEDI®)

Dr. Mary Murphy

Phone: 
+353 91-495206
mary.murphy@nuigalway.ie
Chondrogenic differentiation of stem cells derived from the aorta of an atherosclerotic mouse

Senior Lecturer in Regenerative Medicine; Principal Investigator Orthobiology

Research focus on the Biology, Mechanism of Action and Use of Adult Stem Cells for Tissue Regeneration in Osteoarthritis.

Researcher in

Research Project Area: 

The primary research focus and motivation of the group is the development of innovative medicines to treat major diseases using adult stem cells such as mesenchymal stem/stromal cells (MSCs) or mesenchymal cells derived from induced pluripotent stem cells and gene therapy as a means to provide new modalities in patient care for osteoarthritis, bone repair and vascular calcification. Current clinical research focuses on the therapeutic potential of MSCs in osteoarthritis with a clinical trial in the planning stages. Basic research is focused on the (1) concept that stem cell depletion, or loss/alteration of function, contributes to the development of chronic degenerative diseases such as osteoarthritis and atherosclerosis and (2) the use of the pathway of chondrogenic differentiation of MSCs from bone marrow as a model to study early changes that occur in osteoarthritis development and (3) the role of inflammation in disease progression. Tissue engineering concepts are targeted to cover various aspects of elements required for treatment of osteoarthritis and include 1) development of novel materials for cartilage repair, 2) the use of MSCs and implant surface modification for increased osseointegration of orthopaedic implants used in joint replacement surgeries and 3) the development of targeting strategies and drug delivery modalities for non-invasive delivery of therapeutic cells and/or anti-inflammatory or pro-chondrogenic drugs to osteoarthritic cartilage or to joints using micro- and nano-particles. Applied research has a focus on the economic manufacturing of MSCs for therapeutic use and address novel fully defined serum/medium for cell growth and scale-up for economic manufacture.

Team Members:
Pietro Marchese
Swarna Raman
Claire Dooley
Yvonne Dooley
Georgina Shaw
Dr Jessica Hayes
 

Positions Held: 

2014-present: Senior Lecturer in Regenerative Medicine, NUI, Galway, Ireland

2008-present: Principal Investigator, REMEDI Orthobiology Group, NUI, Galway, Ireland.

2006-2014: Lecturer (Above the Bar) in Regenerative Medicine, NUI Galway, Ireland.

2004-2014: Academic Manager, National Centre of Biomedical Engineering Science Pre-clinical Facility, NUI Galway, Ireland.

2004-2006: Senior Researcher, REMEDI, NUI Galway, Ireland.

2001-2004: Senior Manager, Arthritis Research, Osiris Therapeutics Inc., Baltimore, MD, USA.

1999-2001: Manager, Arthritis Research, Osiris Therapeutics Inc., Baltimore, MD, USA.

Selected Publications: 

https://scholar.google.com/citations?user=Z4xsIQMAAAAJ&hl=en

Selected Publications

Farrell E, Fahy N, Ryan AE, Flatharta CO, O'Flynn L, Ritter T, Murphy JM. vIL-10-overexpressing human MSCs modulate naïve and activated T lymphocytes following induction of collagenase-induced osteoarthritis. Stem Cell Res Ther. 2016 May 18;7(1):74. 

Xu M, Stattin EL, Shaw G, Heinegård D, Sullivan G, Wilmut I, Colman A, Önnerfjord P, Khabut A, Aspberg A, Dockery P, Hardingham T, Murphy M, Barry F. Chondrocytes Derived From Mesenchymal Stromal Cells and Induced Pluripotent Cells of Patients With Familial Osteochondritis Dissecans Exhibit an Endoplasmic Reticulum Stress Response and Defective Matrix Assembly. Stem Cells Transl Med. 2016 Jul 7. pii: sctm.2015-0384. [Epub ahead of print], PMID: 27388238, DOI: 10.5966/sctm.2015-0384.

Leszczynska A, O'Doherty A, Farrell E, Pindjakova J, O'Brien F, O'Brien T, Barry F, Murphy JM. Differentiation of Vascular Stem Cells Contributes to Ectopic Calcification of Atherosclerotic Plaque. Stem Cells. 2016 Apr;34(4):913-23. doi: 10.1002/stem.2315.

Lohan P, Treacy O, Lynch K, Barry F, Murphy M, Griffin MD, Ritter T, Ryan AE. Culture expanded primary chondrocytes have potent immunomodulatory properties and do not induce an allogeneic immune response. Osteoarthritis Cartilage. 2016 Mar;24(3):521-33. doi: 10.1016/j.joca.2015.10.005. PMID: 26493330. Impact Factor 5.093 (5-year).

Fahy N, Farrell E, Ritter T, Ryan AE, JM Murphy. Immune Modulation to Improve Tissue Engineering Outcomes for Cartilage Repair in the Osteoarthritic or Injured Joint. Tissue Eng Part B Rev, 2015 Feb;21(1):55-66. doi: 10.1089/ten.TEB.2014.0098.

Barron V, Neary M, Mohamed KM, Ansboro S, Shaw G, O'Malley G, Rooney N, Barry F, Murphy M. Evaluation of the Early In Vivo Response of a Functionally Graded Macroporous Scaffold in an Osteochondral Defect in a Rabbit Model. Ann Biomed Eng. 2015 Sep;43(9):2069-82. PMID: 26438451.

Sullivan C, Porter R, Evans C, Ritter T, Shaw G, Barry F, Murphy M. "TNFα and IL-1β influence the differentiation and migration of murine mesenchymal stem cells independently of the NF-κB pathway". Stem Cell Res Ther. 2014 Aug 27;5(4):104. PMID: 25163844.

Ansboro S, Hayes JS, Barron V, Browne S, Howard L, Greiser U, Lalor P, Shannon F, Barry FP, Pandit A, Murphy JM. Chondromimetic Microsphere for In Situ Spatially Controlled Chondrogenic Differentiation of Human Mesenchymal Stem Cells. J Control Release. 2014 Apr 10;179:42-51. doi: 10.1016/j.jconrel.2014.01.023. PMID: 24491910.

Barry F, Murphy M. Mesenchymal stem cells in joint disease and repair. Nat Rev Rheumatol. 2013 Oct;9(10):584-94. doi:10.1038/nrrheum. 2013.109. PMID: 23881068.

Sullivan C, Barry FP, Ritter T, O'Flatharta C, Howard L, Shaw G, Anegon I, Murphy JM. Allogeneic murine mesenchymal stem cells: migration to inflamed joints in vivo and amelioration of collagen induced arthritis when transduced to express CTLA4Ig. Stem Cells Dev. 2013 Dec 15;22(24):3203-13. PMID: 23895495.

Ansboro S, Greiser U, Barry F, Murphy M. Strategies for improved targeting of therapeutic cells: implications for tissue repair. Eur Cell Mater. 2012 Apr 21;23:310-8; discussion 318-9.

Mooney E, Mackle JN, Blond DJ, O'Cearbhaill E, Shaw G, Blau WJ, Barry FP, Barron V, Murphy JM. The electrical stimulation of carbon nanotubes to provide a cardiomimetic cue to MSCs. Biomaterials. 2012 Sep;33(26):6132-9.

Mooney E, Dockery P, Greiser U, Murphy M*, Barron V*. Carbon Nanotubes and Mesenchymal Stem Cells: Biocompatibility, Proliferation and Differentiation. Nano Lett. 2008 Aug;8(8):2137-43. Epub 2008 Jul 12. (* Equal contributors).

Research Funding: 

Selected Current Research Funding:

Science Foundation Ireland, Centre for Research in Medical Devices (CÚRAM). Grant No. 13/RC/2073. Research Spoke Project: Mesenchymal Stem/Stromal Cells in Osteoarthritis. 01/01/2017 - 31/12/2018; €500,000; Project principle investigator (PI).

European Union Horizon 2020 PHC16-2015. Autostem: Development of closed, scalable and AUTOmated robotic systems for therapeutic STEM cell manufacturing: aseptic systems from “Donor-to-Patient” (http://www.autostem2020.eu/). Grant no. 667932. 01/01/2016 - 31/12/2018; €849,375 of €5,995,761; Coordinator.

Science Foundation Ireland Investigator Programme H2020 Catalyst Award. Adult and induced pluripotent stem cell-based models to interrogate epigenetic mechanisms in the development of osteoarthritis. Grant no. 15/IACA/3393. €22,900; 01/12/2015 - 30/11/2017. Principle Investigator.

European Union H2020-PHC-2014-2015. ADIPOA2: Clinical trial of autologous adipose-derived mesenchymal stromal cells (ASC) in the treatment of mild to moderate osteoarthritis (http://adipoa2.eu/). Grant No. 643809; 01/01/2015 - 31/12/2018; €250,000 of €5.9M; WP partner.

Irish Blood Transfusion Service: Cell Therapy and Regenerative Medicine. 01/9/2012-31/08/2017; 300,000 of €2,5M; Co-Investigator.

Selected Expired Research Funding:
Science Foundation Ireland: Bone Marrow Stromal Cells in Orthopaedic Applications; Grant No. 09/SRC/B1794: 01/07/2010 – 30/06/2015; €958,448 of 7.456M; Funded Investigator.

EU FP7 Marie Curie Intra-European Fellowship, BIOSTEM: The development of biologically active metal implants for improved osseointegration; Jan 2012 - Sept 2014; €204,587; PI. Fellow, Jessica Hayes, PhD.

EU FP7-NMP-2009-2.3-1: GAMBA: Gene Activated Matrices for Bone and Cartilage Regeneration in Arthritis; Sept 2010 - Aug 2013; €382K. Partner PI.

EU FP7-HEALTH-2007-B: PurStem: Utilisation of the mesenchymal stem cell receptome for rational development of uniform, serum-free culture conditions and tools for cell characterization; Nov 2008 – Oct 2011; €285K; WP leader.
 

Professional and Personal Interests: 

Memberships

International Society for Cell Therapy
International Society for Stem Cell Research
Irish Society for Matrix Biology
Orthopaedic Research Society
Osteoarthritis Research Society International
Tissue Engineering and Regenerative Medicine International Society

Dr Murphy has served on the Scientific Advisory Committee to FES Directorate, Science Foundation Ireland (2006-08) and is a member of the Osteoarthritis Research Society International (OARSI) Communications Committee as well as the Irish national representative on the OARSI Strategic Alliance Committee working with National Partner organisations to advance global osteoarthritis-related "Grand Challenges". She is an Associate Editor of Tissue Engineering and Regenerative Medicine (Frontiers in Bioengineering and Biotechnology) and a reviewer for numerous journals and grant agencies. She is an External Advisory Board for an FP7 NMP project and has been a partner in 4 EU awards as well as a coordinator of the collaborative project Autostem.