The aim of my Research Project is to address the presently unmet need for a cell expansion system that will ultimately be used to produce clinical scale levels of Mesenchymal Stem Cells in a GMP environment. Currently, MSC’s are mainly expanded using the traditional monolayer T-Flask method, due to their attachment-dependent nature. However, only small scale volumes of MSCs can be produced in this way, leading to the need to develop a new system. There are various different expansion routes which I am, and will be carrying out investigations in– both in static and stirred cases. The goal is to choose the best option of cell expansion with the following parameters in mind: Expense, media consumption, time, labour, low contamination risks, capacity for automation etc. The following is a list of some different culture vessels trialled to date: Triple flasks, HYPERflasks, cellSTACKS, roller bottles and Opticells. Future aims are to trial stirred / suspension type expansion systems.